SCIE604 – Molecular Methodologies for Applied Science
Molecular Methodologies for Applied Science
To provide students with knowledge of the principles and methods of molecular biology as applied to Food Technology and Environmental Science.
- Directed hours
- Self directed hours
- Total learning hours
- Learning Outcomes
- Describe the structure and physical properties of nucleic acids and proteins.
Select and conduct appropriate molecular analyses to identify and quantify organisms in a range of food and environmental samples.
Discuss the broad uses and potential of Recombinant DNA Technology, and its application in Food Technology and Environmental Science.
- -Structure and physical properties of DNA, RNA and proteins
-Factors contributing to selection of target molecule for analysis
-DNA, RNA and protein extraction from a range of sample types
-Electrophoresis of nucleic acids and proteins (agarose GE, PAGE)
-Mode of action of restriction enzymes and nucleic acid modifying enzymes
-Nucleic acid hybridization theory
-Methodologies using nucleic acid probes: blotting techniques; microarrays
-Methodologies using nucleic acid primers: PCR; sequencing
-Range nucleic acid fingerprinting/typing methodologies: including RFLP (PFGE), AFLP, DGGE
-Protein analyses: including ELISA, 2D-PAGE, Westerns, FISH
-Use of international nucleic acid and protein databases
-Overview of Recombinant DNA Technology and its application in Food Technology and Environmental Science
-Legislation controlling Recombinant DNA Technology in NZ
- Learning and Teaching Resource
- Learning will be facilitated using a range of teaching and learning methods, which may include:
Practical - laboratory; practical or workshop sessions and activities; demonstrations
Theory - includes web-supported tutor facilitated/directed presentations; lectures and tutorials; group activities; peer presentations and review; group/individual research; online learning activities; case studies; guest speakers
- Assessment Criteria
- Portfolio of Evidence100