SCIE604 – Molecular Methodologies for Applied Science
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Molecular Methodologies for Applied Science
To provide students with knowledge of the principles and methods of molecular biology as applied to Food Technology and Environmental Science.
- Directed hours
- Self directed hours
- Total learning hours
- Learning Outcomes
- Describe the structure and physical properties of nucleic acids and proteins.
Select and conduct appropriate molecular analyses to identify and quantify organisms in a range of food and environmental samples.
Discuss the broad uses and potential of Recombinant DNA Technology, and its application in Food Technology and Environmental Science.
- -Structure and physical properties of DNA, RNA and proteins
-Factors contributing to selection of target molecule for analysis
-DNA, RNA and protein extraction from a range of sample types
-Electrophoresis of nucleic acids and proteins (agarose GE, PAGE)
-Mode of action of restriction enzymes and nucleic acid modifying enzymes
-Nucleic acid hybridization theory
-Methodologies using nucleic acid probes: blotting techniques; microarrays
-Methodologies using nucleic acid primers: PCR; sequencing
-Range nucleic acid fingerprinting/typing methodologies: including RFLP (PFGE), AFLP, DGGE
-Protein analyses: including ELISA, 2D-PAGE, Westerns, FISH
-Use of international nucleic acid and protein databases
-Overview of Recombinant DNA Technology and its application in Food Technology and Environmental Science
-Legislation controlling Recombinant DNA Technology in NZ
- Learning and Teaching Resource
- Learning will be facilitated using a range of teaching and learning methods, which may include:
Practical - laboratory; practical or workshop sessions and activities; demonstrations
Theory - includes web-supported tutor facilitated/directed presentations; lectures and tutorials; group activities; peer presentations and review; group/individual research; online learning activities; case studies; guest speakers
- Assessment Criteria
- Portfolio of Evidence100